FastQCFastQC Report
Sun 14 Apr 2019
SRR6576850_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576850_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1384175
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA55740.40269474596781474No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC34280.247656546318204No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC28750.20770495060234437No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG18640.13466505319052866No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC17080.12339480195784493No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC17020.12296133075658787No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG16900.12209438835407373No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT15020.10851229071468563No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7700.059.8319781
GTATCAA30400.052.577911
TCAACGC35300.044.069844
CAACGCA36500.042.8784945
ATCAACG36650.042.3182953
AACGCAG37600.041.6240736
TATCAAC42650.036.8150562
ACGCAGA46950.033.334727
CGCAGAG47950.032.8355568
GTACATG60400.031.9891641
TAGGTAT10850.031.6214165
TACATGG61550.031.3151022
CTTAGGT11100.030.9092223
GTCTTAG11950.030.68491
ACATGGG63300.030.4416583
TTAGGTA11450.029.9643974
GCAGAGT53050.029.8560719
GGTAATC5450.029.3204468
AGGTATA11500.029.0167436
GTAATCA6550.028.701659
Produced by FastQC (version 0.11.2)