FastQCFastQC Report
Sun 14 Apr 2019
SRR6576851_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576851_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1336050
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA54860.41061337524793234No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC36780.27528909846188393No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC29320.21945286478799444No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC18520.1386175667078328No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG18280.13682122675049588No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC17540.13128251188204035No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG16310.1220762696006886No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT15690.11743572471090154No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT15190.11369334979978295No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7500.064.551371
GTATCAA28550.054.9882431
TCAACGC33050.046.0617564
ATCAACG33850.044.9731523
CAACGCA34950.043.5576825
AACGCAG35650.042.702416
TATCAAC39600.038.932132
ACGCAGA44200.034.4421047
CGCAGAG46050.033.0584378
TAGGTAT11900.032.7716335
CTTAGGT12450.032.4560783
GTACATG54350.032.4310651
TACATGG55600.031.6174132
TTAGGTA13250.031.5602974
GTCTTAG13650.031.3356171
AGGTATA12450.030.9464936
ACATGGG56250.030.8227083
GCAGAGT50850.030.0302779
ACCTAAG18900.028.8486651
TCTTAGG15900.028.6750472