FastQCFastQC Report
Sun 14 Apr 2019
SRR6576851_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576851_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1336050
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA57570.4308970472661951No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC36690.2746154709778826No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC28980.21690804984843384No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG17240.12903708693536917No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC17090.1279143744620336No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC16410.12282474458291232No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG15490.1159387747464541No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT15190.11369334979978295No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT14980.11212155233711313No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG14020.10493619250776542No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7200.064.086761
GTATCAA29350.055.025091
TCAACGC34750.045.0316774
ATCAACG35600.043.8244823
CAACGCA36000.043.7291455
AACGCAG37600.041.866766
TATCAAC41050.038.5813942
CGCAGAG46150.034.1332978
ACGCAGA46500.033.8535587
GGTAATC4350.033.5102428
GTCTTAG12650.033.4986081
CTTAGGT11600.032.813743
TAGGTAT12000.032.5031555
TTAGGTA12500.031.9549084
GTAATCA4900.031.6681699
GTACATG55050.031.0473141
GCAGAGT51150.030.8886389
TACATGG56350.030.5244872
TCTTAGG14550.030.0387022
AGGTATA12900.029.8700926