FastQCFastQC Report
Sun 14 Apr 2019
SRR6576854_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576854_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences959279
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA145561.517389622831314No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC97571.0171180647131857No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC74150.7729763707951492No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG54340.5664671070668701No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC50540.5268540226565994No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC43440.4528401017847779No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT39090.4074935446309155No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA37760.39362896508732076No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA36200.37736675148731497No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT35440.3694441346052608No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT35310.36808895013859366No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC34810.3628767021898739No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA32610.3399428112155066No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC32430.33806640195396753No Hit
ACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT31250.32576549679498873No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT28770.29991274696933845No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT26190.27301754755394414No Hit
ACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATT25710.2680137895231731No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA24020.25039639145650017No Hit
GCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCA24010.25029214649752574No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG23950.24966667674367937No Hit
GTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTT23900.24914545194880738No Hit
GTACATGGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAG22680.23642756695393102No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG22220.23163229884110878No Hit
GACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGA21930.2286091950308513No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACA20490.2135979209385382No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT20400.21265971630776864No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTT20320.21182575663597347No Hit
GTTTGGGGCTGACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACG19540.20369464983597055No Hit
ATTCAATACATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGG19180.19994183131289228No Hit
ACTATGTGAGGAGCATGGAATCCTTAGAGAAAATATCATTGACCTATCCA18730.19525080815904444No Hit
GATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACAC18280.1905597850051966No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG18170.18941309045647825No Hit
GTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCCATTCTTCA17850.18607725176929757No Hit
GTACATGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGA17720.1847220673026304No Hit
GACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAA17710.18461782234365604No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTTCTCACATAGTT17630.18378386267186084No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTTCTCACA17600.18347112779493766No Hit
ATCATAGTCTGTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCA17160.17888434960006422No Hit
GACCTATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGC16980.17700794033852507No Hit
GGATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGG16650.17356785669237002No Hit
GGGTATATCAAAGAGAAATTTAAAGAAGTCTTTGATTCTTGATGAGGTAT16640.1734636117333956No Hit
CTCCAGGCCCGAGAATGAAGAATGGCCTGAGCCTCCAGTGTTGAGTGGAG16640.1734636117333956No Hit
TACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTA16240.16929381337441976No Hit
ATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCC16130.16814711882570138No Hit
GGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTC15800.1647070351795463No Hit
ACTCAACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAG15790.1646027902205719No Hit
CTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCAC15740.1640815654256999No Hit
GAATTATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGT15610.16272638095903277No Hit
GGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCCATTCTTCATTCTC15240.1588693174769801No Hit
GGATGGGGAAACCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATT14990.1562631935026202No Hit
GGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAG14760.15386555944620908No Hit
GGTGAATATTCTGTGACGTATGATGGATTCAATACATTTACTATACCTAA14620.15240613002056755No Hit
GCATACTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATG14260.14865331149748925No Hit
GATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACAGAAAAGGC13970.14563020768723176No Hit
CTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGAAA13690.14271134883594866No Hit
GTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTCTCGGCCA13650.14229436900005107No Hit
GTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTCTCGGCCATAG13320.138854285353896No Hit
CTGTAAGAGATGAAGAGTGCTCGGAATTATCTATGGTTGCTGACAAAACA13200.13760334584620323No Hit
CAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAAT12940.13489297691286894No Hit
TCATTGACCTATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGA12740.13280807773338102No Hit
TCACAGAATATTCACCAGCCTTTTCTGTTTTGTCAGCAACCATAGATAAT12670.13207836302056025No Hit
GAGGTATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGA12550.13082742351286747No Hit
GTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAAGATGCTGCTG12550.13082742351286747No Hit
GTATAAATTCTGTGATCTGCATTCCATCCTGTCTCACTGAGAAGTCCAAT12140.12655338019491724No Hit
CCTCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGT12080.12592791044107085No Hit
GTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTTTAATGT11910.1241557461385061No Hit
GGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTTGTGCA11830.12332178646671094No Hit
ATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGGAA11800.12300905158978774No Hit
ATACATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCAT11620.12113264232824861No Hit
CCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTTT11620.12113264232824861No Hit
CATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTC11500.11988170282055585No Hit
ATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTG11450.11936047802568388No Hit
CTATGGCCGAGAACCAGATTTGAGTTCAGACATCAAGGAAAGGTTTGCAC11410.11894349818978628No Hit
GATTAATGGGGAATGGCATACTATTATCCTGGCCTCTGACAAAAGAGAAA11340.11821378347696551No Hit
GGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGA11260.11737982380517033No Hit
GTAAATGTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTC11200.11675435405132396No Hit
GGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTCACCAGC11150.11623312925645199No Hit
ATTCTGTGACGTATGATGGATTCAATACATTTACTATACCTAAGACAGAC11090.1156076595026056No Hit
ATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGAAACC11090.1156076595026056No Hit
ACTTAAGACAGACTATGATAATTATATTATGATTCATCTCATTAACAAAA10880.11341851536414327No Hit
ATATTCACCAGCCTTTTCTGTTTTGTCAGCAACCATAGATAATTCGGAGC10830.1128972905692713No Hit
CAGTGAGACAGGATGGAATGCAGATCACAGAATTTATACTGGGGATGCTG10810.1126888006513225No Hit
ATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAACTTTAG10720.11175059602055294No Hit
CTCTTACAGTATGGACTTTAAGAACTAAGGAATTCTCCAAGACATGGATT10670.11122937122568097No Hit
TAGTAAATGTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTT10550.10997843171798818No Hit
CCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATTTGAGTTCAGAC10550.10997843171798818No Hit
GTATGATGGATTCAATACATTTACTATACCTAAGACAGACTATGATAACT10500.10945720692311621No Hit
CCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTTGTCAGCAAC10470.10914447204619304No Hit
GTATCAACGCAGAGTACATGGGGGAGTGTAGCCACGATCACAAGAAAGAC10460.10904022708721862No Hit
ACTATGTGAGAAGCATGGAATCCTTAGAGAAAATATCATTGACCTATCCA10450.10893598212824422No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10400.10841475733337225No Hit
GGTCAATGATATTTTCTCTAAGGATTCCATGCTTCTCACATAGTTGTGCA10080.10507891864619157No Hit
GGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACA9950.10372373417952441No Hit
CTCCAAGACATGGATTTGCTCCAGAAAAAGTCTAAAGTTGCCATTATCTT9870.10288977450772924No Hit
TCACAGAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTG9770.10184732491798527No Hit
AATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCC9720.1013261001231133No Hit
AGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGG9700.10111761020516451No Hit
TCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTATACTGGGGATG9690.1010133652461901No Hit
GTCTTTGATTCTTGATGAGGTATCCTAGGTAACATGTGGATAGACTGGAA9600.10007516061542054No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5800.068.084671
GTGCAAG8000.051.1242941
ATCAACG28200.048.998533
TCAACGC28400.048.6534734
CAACGCA28900.048.1369675
TATCAAC29350.047.7316252
AACGCAG29450.047.2379726
AAGGGTA8350.044.4657335
GTATCAA33250.043.688271
TGCAAGG8600.042.637742
ACGCAGA34750.040.0333337
CGCAGAG35000.039.61318
AGGGTAA9650.038.4755336
CTTAGGT25550.038.2610863
TTAGGTA25700.038.0377734
TAGGTAT25700.037.8548975
GTCTTAG25950.037.681141
TAGGCCG500.001702137337.5988735
GCAAGGG9900.037.503933
TCTTAGG26500.037.4312742
Produced by FastQC (version 0.11.2)