FastQCFastQC Report
Sun 14 Apr 2019
SRR6576854_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576854_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences959279
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA153711.602349264395447No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC93560.9753158361644526No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC74820.7799607830464338No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG46300.48265416005145534No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC42780.4459599344924678No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC41940.4372033579386185No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG38340.3996751727078358No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT37900.39508839451296235No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT36800.3836214490257787No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC34120.3556838000206405No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT33560.3498460823180743No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA33060.34463383436935446No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA31040.3235763526565264No Hit
ACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT30670.31971928917447373No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA29650.3090863033590853No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC29380.30627168946677663No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT28920.30147642135395436No Hit
GACCTATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGC28200.29397078430779783No Hit
ACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATT24430.2546704347744504No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA24400.2543576998975272No Hit
GACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGA23540.24539263342572912No Hit
GTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTT23020.23997189555906048No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT22880.23851246613341895No Hit
GTACATGGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAG22500.2345511576923919No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG21280.22183327269751554No Hit
ACTATGTGAGGAGCATGGAATCCTTAGAGAAAATATCATTGACCTATCCA21110.22006110839495077No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACA20580.2145361255693078No Hit
GCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCA20290.21151302175905026No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTT20110.20963661249751114No Hit
GTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCCATTCTTCA19630.2046328544667401No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT19410.20233946536930342No Hit
TCATTGACCTATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGA18770.19566778799494203No Hit
GTTTGGGGCTGACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACG18630.19420835856930047No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG18190.18962158037442706No Hit
ATTCAATACATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGG17840.1859730068103232No Hit
CTCCAGGCCCGAGAATGAAGAATGGCCTGAGCCTCCAGTGTTGAGTGGAG17280.18013528910775697No Hit
GTACATGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGA17160.17888434960006422No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTTCTCACA17090.17815463488724342No Hit
GATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACAC16940.1765909605026275No Hit
TACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTA16850.17565275587185794No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTTCTCACATAGTT16730.17440181636416516No Hit
ATCATAGTCTGTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCA16590.17294238693852362No Hit
ACTCAACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAG15670.16335185071287914No Hit
ATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCC15620.16283062591800718No Hit
GAATTATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGT15150.15793111284621053No Hit
GGTGAATATTCTGTGACGTATGATGGATTCAATACATTTACTATACCTAA15050.15688866325646658No Hit
GGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCCATTCTTCATTCTC14990.1562631935026202No Hit
GGATGGGGAAACCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATT14560.15178066026672116No Hit
CATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTC14450.15063396571800278No Hit
GGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAG14440.1505297207590284No Hit
CTGTAAGAGATGAAGAGTGCTCGGAATTATCTATGGTTGCTGACAAAACA14410.15021698588210522No Hit
GGATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGG14390.1500084959641564No Hit
CTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGAAA14360.14969576108723323No Hit
GACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAA14330.14938302621031002No Hit
CTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCAC14200.14802784174364286No Hit
GGGTATATCAAAGAGAAATTTAAAGAAGTCTTTGATTCTTGATGAGGTAT14090.14688114719492454No Hit
GCATACTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATG13910.1450047379333854No Hit
CAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAAT13530.14104342949235832No Hit
GATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACAGAAAAGGC13480.14052220469748636No Hit
GTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTCTCGGCCA13290.1385415504769728No Hit
CTATGGCCGAGAACCAGATTTGAGTTCAGACATCAAGGAAAGGTTTGCAC13180.13739485592825446No Hit
GGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTC13130.13687363113338247No Hit
GTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTTTAATGT12910.13458024203594574No Hit
ATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGAAACC12430.1295764840051747No Hit
TCACAGAATATTCACCAGCCTTTTCTGTTTTGTCAGCAACCATAGATAAT12420.12947223904620034No Hit
GTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAAGATGCTGCTG12130.12644913523594284No Hit
ACTATGTGAGAAGCATGGAATCCTTAGAGAAAATATCATTGACCTATCCA12120.12634489027696844No Hit
ATACATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCAT12030.12540668564619886No Hit
CCTCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGT11890.1239472562205573No Hit
GAGGTATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGA11730.12227933687696697No Hit
GTATAAATTCTGTGATCTGCATTCCATCCTGTCTCACTGAGAAGTCCAAT11700.12196660200004378No Hit
GTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTCTCGGCCATAG11570.12061141753337663No Hit
CCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTTT11520.12009019273850464No Hit
GTAAATGTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTC11370.11852651835388871No Hit
GGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTTGTGCA11210.11685859901029835No Hit
GGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTCACCAGC11160.11633737421542636No Hit
ATTCTGTGACGTATGATGGATTCAATACATTTACTATACCTAAGACAGAC11140.11612888429747759No Hit
CTCTTACAGTATGGACTTTAAGAACTAAGGAATTCTCCAAGACATGGATT11050.11519067966670803No Hit
TAGTAAATGTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTT11030.11498218974875922No Hit
ATATTCACCAGCCTTTTCTGTTTTGTCAGCAACCATAGATAATTCGGAGC10950.11414823007696406No Hit
ATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGGAA10930.11393974015901526No Hit
CCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATTTGAGTTCAGAC10920.11383549520004087No Hit
ACTTAAGACAGACTATGATAATTATATTATGATTCATCTCATTAACAAAA10790.11248031073337371No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10740.11195908593850173No Hit
CCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTTGTCAGCAAC10710.11164635106157854No Hit
GATTAATGGGGAATGGCATACTATTATCCTGGCCTCTGACAAAAGAGAAA10630.11081239138978337No Hit
GGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGA10370.10810202245644908No Hit
GTATCAACGCAGAGTACATGGGGGAGTGTAGCCACGATCACAAGAAAGAC10190.10622561319490993No Hit
ATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAACTTTAG10170.10601712327696113No Hit
TTACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATT10140.10570438840003796No Hit
TCCCCATCCTTTTCGTTAATGAGATGAGCCATAAGAAAGTTATCATAGTC9980.1040364690564476No Hit
CTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGAAACCTTCCAGC9890.10309826442567803No Hit
GTATGATGGATTCAATACATTTACTATACCTAAGACAGACTATGATAACT9840.10257703963080604No Hit
ATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTTCCCGT9680.10090912028721571No Hit
CTCCAAGACATGGATTTGCTCCAGAAAAAGTCTAAAGTTGCCATTATCTT9660.10070063036926692No Hit
CAGTGAGACAGGATGGAATGCAGATCACAGAATTTATACTGGGGATGCTG9600.10007516061542054No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5700.062.827211
AAGGGTA6150.057.3171355
GTGCAAG6600.054.9738041
TGCAAGG6650.050.887272
AGGGTAA7500.047.000056
GCAAGGG7300.046.3562163
GGTAATC7650.045.461738
GTAATCA8700.042.135789
GTATCAA27550.041.7327771
CAAGGGT8900.041.7191474
ATCAACG27300.040.9744033
CAACGCA27650.040.7957085
TATCAAC27300.040.6300772
TCAACGC27550.040.6025854
AACGCAG28350.039.954196
GGGTAAT9650.036.039617
GTACATG44800.035.1299481
TAGGTAT25900.034.2973375
GTACTAG550.002682913834.2693861
CTTAGGT26350.033.889983
Produced by FastQC (version 0.11.2)