FastQCFastQC Report
Sun 14 Apr 2019
SRR6576855_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576855_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences943357
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA152781.6195353402794488No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC95501.0123420931842346No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC73000.7738321759418756No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG45420.48147201960657526No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC42280.44818663560030825No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG41730.44235639317882836No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC41650.4415083579175222No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT38230.40525485049668364No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT37660.39921259925987723No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC34230.3628530874313754No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT34130.36179304335474266No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA33120.35108659818075233No Hit
ACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT31000.328613663756139No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA30470.32299543014998566No Hit
GACCTATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGC30290.32108735081204676No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC29490.3126069981989851No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT29080.308260817484791No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA28790.3051866896625562No Hit
ACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATT24280.25737870180642114No Hit
GTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTT23930.25366854753820667No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA23530.2494283712316758No Hit
GACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGA22950.2432801155872061No Hit
ACTATGTGAGGAGCATGGAATCCTTAGAGAAAATATCATTGACCTATCCA22800.24169004947225706No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT21440.22727345003005225No Hit
TCATTGACCTATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGA21290.22568338391510318No Hit
GTACATGGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAG20880.2213372032009091No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACA20870.22123119879324582No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG20590.21826307537867423No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT20370.21593097841008227No Hit
GCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCA20300.2151889475564394No Hit
CTCCAGGCCCGAGAATGAAGAATGGCCTGAGCCTCCAGTGTTGAGTGGAG18950.20087835252189787No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTT18420.19526011891574452No Hit
GTTTGGGGCTGACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACG18170.19261000872416276No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG17730.18794581478697883No Hit
TACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTA17710.18773380597165232No Hit
ATTCAATACATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGG17660.18720378393333595No Hit
GTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCCATTCTTCA17440.18487168696474401No Hit
GTACATGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGA17010.18031349743522335No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTTCTCACA16790.1779814004666314No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTTCTCACATAGTT16670.17670934757467216No Hit
ATCATAGTCTGTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCA16370.17352921534477406No Hit
GAATTATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGT16270.17246917126814132No Hit
ACTCAACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAG16070.17034908311487593No Hit
GATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACAC15650.16589689799301854No Hit
GTATAAATTCTGTGATCTGCATTCCATCCTGTCTCACTGAGAAGTCCAAT15250.1616567216864877No Hit
GACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAA15160.16070268201751828No Hit
GGATGGGGAAACCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATT14890.15784056301060997No Hit
GGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCCATTCTTCATTCTC14560.15434241755772204No Hit
CTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGAAA14550.1542364131500588No Hit
GGTGAATATTCTGTGACGTATGATGGATTCAATACATTTACTATACCTAA14340.1520103205891301No Hit
CATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTC14320.15179831177380357No Hit
GGATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGG14100.1494662148052116No Hit
ATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCC14080.14925420598988506No Hit
GATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACAGAAAAGGC13840.14671010020596656No Hit
GTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTCTCGGCCA13820.14649809139064002No Hit
CTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCAC13750.14575606053699713No Hit
TCACAGAATATTCACCAGCCTTTTCTGTTTTGTCAGCAACCATAGATAAT13570.14384798119905826No Hit
GCATACTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATG13570.14384798119905826No Hit
GGGTATATCAAAGAGAAATTTAAAGAAGTCTTTGATTCTTGATGAGGTAT13540.14352996797606846No Hit
CTGTAAGAGATGAAGAGTGCTCGGAATTATCTATGGTTGCTGACAAAACA13520.14331795916074191No Hit
GGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTC13270.14066784896916015No Hit
CAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAAT13130.13918378726187436No Hit
ACTATGTGAGAAGCATGGAATCCTTAGAGAAAATATCATTGACCTATCCA13070.13854776081589473No Hit
GGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAG12800.13568564180898643No Hit
CTATGGCCGAGAACCAGATTTGAGTTCAGACATCAAGGAAAGGTTTGCAC12660.1342015801017006No Hit
ATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGAAACC12620.13377756247104755No Hit
GTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTCTCGGCCATAG12610.13367155806338427No Hit
GTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTTTAATGT12530.13282352280207813No Hit
GGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTCACCAGC12080.12805332445723094No Hit
ATACATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCAT11870.12582723189630224No Hit
CTCTTACAGTATGGACTTTAAGAACTAAGGAATTCTCCAAGACATGGATT11790.12497919663499608No Hit
GAGGTATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGA11760.12466118341200626No Hit
GGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTTGTGCA11730.12434317018901646No Hit
GTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAAGATGCTGCTG11550.12243509085107758No Hit
CCTCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGT11480.12169305999743468No Hit
GTAAATGTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTC11480.12169305999743468No Hit
CCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATTTGAGTTCAGAC11420.12105703355145506No Hit
ATTCTGTGACGTATGATGGATTCAATACATTTACTATACCTAAGACAGAC11380.12063301592080199No Hit
TAGTAAATGTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTT11290.11967897625183256No Hit
CCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTTGTCAGCAAC11250.11925495862117946No Hit
CCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTTT11150.11819491454454675No Hit
ATATTCACCAGCCTTTTCTGTTTTGTCAGCAACCATAGATAATTCGGAGC10860.11512078672231191No Hit
GGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGA10630.11268268534605667No Hit
CTCCAAGACATGGATTTGCTCCAGAAAAAGTCTAAAGTTGCCATTATCTT10610.11247067653073015No Hit
GATTAATGGGGAATGGCATACTATTATCCTGGCCTCTGACAAAAGAGAAA10460.11088061041578108No Hit
ACTTAAGACAGACTATGATAATTATATTATGATTCATCTCATTAACAAAA10300.10918453989316876No Hit
ATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAACTTTAG10200.10812449581653605No Hit
TCCCCATCCTTTTCGTTAATGAGATGAGCCATAAGAAAGTTATCATAGTC10080.1068524429245768No Hit
ATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGGAA10040.10642842529392373No Hit
TTACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATT9920.10515637240196447No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9910.1050503679943012No Hit
TCTTATGGCTCATCTCATTAACGAAAAGGATGGGGAAACCTTCCAGCTGA9880.1047323547713114No Hit
GTATCAACGCAGAGTACATGGGGGAGTGTAGCCACGATCACAAGAAAGAC9810.10399032391766849No Hit
ATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTTCCCGT9740.1032482930640256No Hit
GTATGATGGATTCAATACATTTACTATACCTAAGACAGACTATGATAACT9480.10049217846478056No Hit
ATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTG9440.10006816083412749No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4950.077.937171
GTGCAAG5350.059.798621
AAGGGTA5950.049.7594835
GTACTAG502.991126E-547.0474361
GTATCAA27450.045.5905951
ATCAACG26150.045.108083
TCAACGC26400.044.680924
CAACGCA26300.044.4934275
TGCAAGG6800.044.230652
GGTAATC6950.043.9801988
TATCAAC27150.043.965922
AACGCAG27000.043.1635556
GTGTAAT2300.042.9563561
AGGGTAA7400.040.642236
GCAAGGG7550.039.2144283
GTAATCA8450.037.8425879
TAGGCCG500.001702749937.5960545
ACGCAGA31800.036.6483047
CGCAGAG32100.036.477328
TAGGTAT25750.036.1360175
Produced by FastQC (version 0.11.2)