FastQCFastQC Report
Sun 14 Apr 2019
SRR6576857_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576857_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences924834
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA37020.40028805169360127No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC24050.2600466678344438No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC22860.24717949383348797No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC15580.16846266465116985No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC14780.1598124636421239No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT12150.13137492782488533No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT10670.11537205595815034No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10580.11439890834463266No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG10520.11375014326895422No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA9640.10423492215900367No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4150.062.2984731
GTATCAA19000.055.6662251
TCAACGC22750.045.850764
ATCAACG23150.045.058523
CAACGCA23500.044.3874365
AACGCAG24250.043.208396
TATCAAC26850.039.3913652
TAGGTAT7350.037.7172135
GTCTTAG8200.036.6884161
AGGTATA7450.035.9495546
CTTAGGT7500.035.7098923
ACGCAGA29550.035.458667
TTAGGTA8050.034.4374584
CGCAGAG31200.033.7340438
GGTATAG8250.032.463547
GTATAGT9700.032.454738
GTACATG38800.031.9841651
TACATGG39000.031.8201452
TATAGTA10050.031.3244679
GGTAATC3450.031.3244658