FastQCFastQC Report
Sun 14 Apr 2019
SRR6576857_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576857_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences924834
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA37500.40547817229902877No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC24300.26274985564977066No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC22700.24544945363167875No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC14040.15181102770875637No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC13030.14089014893483587No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT12530.13548377330418215No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11410.12337349189151783No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT10420.11266886814282347No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG10250.1108307004284012No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG9470.10239675444458142No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5500.061.633671
GTATCAA19650.054.628661
TCAACGC23700.044.8135384
ATCAACG24050.044.1613663
CAACGCA24550.043.4533735
AACGCAG25300.042.3486946
TAGGTAT7100.040.3757485
TATCAAC26750.039.7082522
CTTAGGT7400.038.1038363
TTAGGTA7950.036.6499754
AGGTATA7800.036.147846
GTACATG38500.035.586111
ACGCAGA30150.035.380527
GTCTTAG8850.035.1114651
TACATGG38900.035.038422
GGTATAG8200.034.384537
ACATGGG39650.034.1348843
CGCAGAG31300.034.104578
GTATAGT9700.032.481358
TATAGTA9750.032.3147779