FastQCFastQC Report
Sun 14 Apr 2019
SRR6576858_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576858_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences495998
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG12510.25221875894660867No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA7430.1497989911249642No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6590.1328634389654797No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6210.1252021177504748No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5800.11693595538691688No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5740.11572627308981083No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5530.11149238504993972No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5390.10866979302335895No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT5370.10826656559099028No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC5310.10705688329388426No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC4960.10000040322743237No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12750.047.5855561
TCAACGC15950.037.4224364
ATCAACG16450.036.5706833
CAACGCA16200.036.554815
AACGCAG16450.035.9992686
GTACATG26250.032.6090161
TACATGG26350.032.4852642
GGGTACC4350.032.413137
ACATGGG26950.031.9140053
TGGGTAC4700.029.9993886
TATCAAC20500.029.825322
ACGCAGA19900.029.7581867
GTACTAG953.730524E-529.7045691
CGCAGAG20000.029.6093968
GGTACCT4800.029.37448
ATGGGTA5250.028.6470385
CATGGGG14100.027.999434
GGTATCA6150.027.5310671
ATGGGGG4800.027.4161075
CATGGGT6900.027.2458234