FastQCFastQC Report
Sun 14 Apr 2019
SRR6576858_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576858_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences495998
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11000.22177508780277339No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8100.16330711010931495No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6780.13669409957298215No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6660.13427473497877007No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA6020.12137145714297234No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5880.1185488651163916No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5490.11068593018520236No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA5400.10887140673954329No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG5280.10645204214533123No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5210.10504074613204084No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA5190.10463751869967217No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC5070.10221815410546012No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT5040.10161331295690708No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12950.044.3237841
TACATGG26800.035.0719572
GTACATG26900.034.805471
ACATGGG27200.034.383413
CAACGCA16450.033.997415
TCAACGC16500.033.8943864
ATCAACG16650.033.5890353
ATGGGTA4900.032.609765
AACGCAG17150.032.609766
GGTACCT4400.032.0430158
GGGTACC4450.031.682987
GGTATCA6700.031.5998061
CATGGGG14350.031.4401134
TGGGTAC4900.028.773326
ATTATCC3700.027.9438173
TATCAAC20950.027.8164962
ACGCAGA20250.027.617657
TTAGGGA1057.417055E-526.8550994
ACTAGAG1057.417055E-526.8550992
ATGGGAG5150.026.4640035
Produced by FastQC (version 0.11.2)