FastQCFastQC Report
Sun 14 Apr 2019
SRR6576859_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576859_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1149025
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA44340.38589238702378104No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC23810.20721916407388874No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC20580.17910837449141664No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG15300.13315637170644679No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA13950.12140728008528971No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA13010.1132264311046322No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC12930.11253018863819325No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT12660.11018037031396183No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG12500.10878788538108396No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG12230.10643806705685256No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG12110.10539370335719414No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG11920.10374012749940166No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG11780.10252170318313353No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT11770.10243467287482866No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7900.059.0702821
GTATCAA26650.053.062171
TCAACGC31100.044.583424
ATCAACG31800.043.6020243
CAACGCA32550.042.5973635
AACGCAG33700.041.5621576
TATCAAC37600.037.3761942
ACGCAGA40350.034.3614357
TAGGTAT7750.033.9623765
GTCTTAG8650.032.696111
CGCAGAG42600.032.4348348
ACCTAAG14150.030.980431
AGGTATA8200.030.952216
CTTAGGT8050.030.9450863
GTACATG51350.030.8432271
GCAGAGT45650.030.3707249
TACATGG51900.030.2475932
GTTATAC1102.9677049E-629.9100483
GGTATAG8550.029.6838677
CTAAGAC15050.029.3564193
Produced by FastQC (version 0.11.2)