FastQCFastQC Report
Sun 14 Apr 2019
SRR6576860_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576860_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1154157
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA46710.4047109708644491No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC25860.22405963833343295No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC22520.19512076779848841No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG14800.12823212093328723No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA14090.12208044486148764No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC13630.11809485191356114No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG12950.11220310581662633No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG12810.11099009926725739No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG12590.10908394611824908No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT12360.10709114964428583No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA12230.10596478641987181No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT11930.1033654866712241No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG11910.10319220002131427No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11890.10301891337140441No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG11870.10284562672149455No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT11850.10267234007158473No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7200.068.684711
GTATCAA26500.055.9845161
ATCAACG31800.045.9575733
TCAACGC31900.045.81354
CAACGCA32400.044.9614685
AACGCAG33100.044.010626
TATCAAC38750.037.8394052
ACGCAGA39750.036.5296177
GTCTTAG8750.034.987151
CGCAGAG41550.034.9698338
TAGGTAT8050.033.8575825
TTAGGTA8250.033.606394
CTTAGGT8100.033.0684363
GGTAATC3850.032.9768458
GCAGAGT44200.032.7668389
GTACATG48750.031.785171
TACATGG49650.031.236052
TCTTAGG10250.031.1778722
AGGTATA8700.030.2477156
ACATGGG50650.030.1528033
Produced by FastQC (version 0.11.2)