FastQCFastQC Report
Sun 14 Apr 2019
SRR6576861_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576861_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1336488
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA58100.43472144905154403No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC36640.2741513578872388No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC28510.2133202842075649No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG18620.13932036800929No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC18560.1388714301961559No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG17880.13378346831396914No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC17600.1316884251860099No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT16260.12166214735934777No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT14020.10490180233567378No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA14000.10475215639796241No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT13780.10310605108313729No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT13540.1013102998306008No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7700.066.5476151
GTATCAA28650.051.3589861
TCAACGC32750.043.7694934
ATCAACG33150.043.383133
CAACGCA33700.042.8145565
AACGCAG34350.042.141216
TATCAAC38500.037.364352
TAGGTAT11700.033.7424355
CTTAGGT12000.032.8988723
GTCTTAG13300.032.8721471
ACGCAGA44750.032.3474967
TTAGGTA12350.032.3470734
CGTATAG753.2714542E-431.3404661
CGGCGAT753.2764827E-431.3322694
GTACATG54550.031.2830161
CGCAGAG46650.031.2315148
TCTTAGG15000.031.0270632
TACATGG54050.030.963572
ACATGGG55950.030.072253
GTATAGT14450.029.9228528
Produced by FastQC (version 0.11.2)