FastQCFastQC Report
Sun 14 Apr 2019
SRR6576862_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576862_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1280029
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA58950.4605364409712593No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC37930.29632141146802143No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC28440.22218246617850065No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC19560.15280903792023462No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC18480.14437172907801307No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG18130.14163741602729313No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT16900.13202825873476304No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG16670.13023142444428992No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT15340.11984103485155415No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA13960.1090600291087155No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA13280.10374764946731675No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT13270.1036695262372962No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC13140.10265392424702877No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT13050.10195081517684365No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6650.057.960381
GTATCAA27000.054.490351
TCAACGC32150.045.305414
ATCAACG32750.044.4753843
CAACGCA33200.043.872565
AACGCAG34850.041.9302026
TATCAAC37600.038.6286622
TAGGTAT11150.037.9259955
GTCTTAG13000.037.6035651
TTAGGTA11300.037.4225544
CTTAGGT11250.037.171223
ACGCAGA42250.034.4750027
AGGTATA12300.034.380076
CGCAGAG43200.033.7168778
TATAGTA14050.033.107649
GGTATAG12650.033.0574077
GTACATG52700.032.287691
TCTTAGG15300.031.950742
TACATGG53800.031.3654272
GTATAGT14900.031.2189468
Produced by FastQC (version 0.11.2)