FastQCFastQC Report
Sun 14 Apr 2019
SRR6576862_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576862_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1280029
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA60700.474208006224859No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC36930.28850908846596446No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC29190.22804170843004337No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC18280.14280926447760167No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC16400.12812209723373455No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG16160.1262471397132409No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG15890.12413781250268548No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT15250.11913792578136902No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT14770.11538801074038167No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG14090.11007563109898293No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT14060.1098412614089212No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT13990.10929439879877723No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC13790.10773193419836581No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5700.066.879751
GTATCAA26400.055.442271
TCAACGC30500.046.9918374
ATCAACG31050.046.159453
CAACGCA31550.045.725815
AACGCAG32350.044.740296
TATCAAC36000.040.2041242
GTATACG500.001690842637.650831
ACGCAGA40500.035.7377
CGCAGAG41850.034.4961668
TAGGTAT11050.033.1707085
CTTAGGT11500.031.8727263
GTACATG53700.031.8138961
GCAGAGT45550.031.7972989
TACATGG54200.031.125592
TTAGGTA11850.030.9313344
GTCTTAG13550.030.5652481
ACATGGG55450.030.2544363
AGGTATA12300.029.79976
GTATAGT13850.028.8600468
Produced by FastQC (version 0.11.2)