FastQCFastQC Report
Sun 14 Apr 2019
SRR6576865_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576865_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1157804
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA52870.45664032945127153No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC36030.3111925679994196No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC31270.2700802553800125No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG19630.1695451043527229No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC17930.15486213556007752No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG15800.1364652393669395No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT15340.132492200752459No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA13910.12014123288570432No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA13470.11634093508054903No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC13400.11573634224791071No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA13370.11547723103392284No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC12680.10951767311220206No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT12460.10761752420962442No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT12310.10632196813968513No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA12170.10511278247440844No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG12100.10450818964177011No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA11930.10303989276250557No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT11880.10260804073919247No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7550.069.7288741
GTATCAA30000.049.5116461
TCAACGC33100.043.8748634
ATCAACG33450.043.5562863
CAACGCA34550.042.0335165
AACGCAG35350.041.0822646
TATCAAC38650.038.0659262
CTTAGGT12000.033.6823583
TAGGTAT12200.033.1301885
ACGCAGA44200.032.9628457
TTAGGTA12950.031.9372984
CGCAGAG45700.031.8809178
AGGTATA12600.031.7054316
GTCTTAG12900.031.7008631
GGTATAG13200.031.6884757
GTACATG53000.031.6616761
TCTTAGG13900.031.1110442
TACATGG54500.030.531512
ACATGGG55500.029.8081473
GCAGAGT49450.029.4632539