FastQCFastQC Report
Sun 14 Apr 2019
SRR6576866_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576866_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1076745
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA53370.4956605324380425No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC37280.3462286799567214No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC29000.2693302499663337No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC16350.1518465374810192No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG15920.14785301998151837No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT13870.1288141574838982No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG13800.12816404998397948No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG13710.12732819748408397No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC13700.1272353249840956No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA12770.11859818248517523No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA12020.1116327449860459No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT11660.10828933498646383No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC11640.10810358998648704No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT11480.10661762998667279No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC11460.10643188498669601No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT11170.10373858248703269No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT11160.10364570998704428No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC10910.10132389748733452No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6500.060.090481
GTATCAA24450.050.2345541
TCAACGC26250.045.1135144
ATCAACG26300.045.027753
CAACGCA27750.042.5055965
AACGCAG28600.041.404716
TATCAAC31000.038.3578952
AAGGGTA3950.035.6910675
ACGCAGA35000.033.6993037
GGTAATC4150.032.858498
GTCTTAG12500.032.752931
CGCAGAG36250.032.5584268
TAGGTAT12150.032.4891555
CTTAGGT11750.032.395343
TTAGGTA12100.031.8466634
GTACATG47000.031.038791
GTGCAAG5550.030.5246321
TACATGG47100.030.3353212
AGGTATA12750.030.22176
TCTTAGG13400.030.1640432
Produced by FastQC (version 0.11.2)