FastQCFastQC Report
Sun 14 Apr 2019
SRR6576867_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576867_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1320847
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA43200.3270628619363181No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC26100.1976004790865255No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC22270.16860393368800475No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG16070.1216643562804776No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG15020.11371491171952543No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA14800.11204931381151641No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA14510.10985375293277723No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC13880.10508408619620592No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC13760.10417557824638282No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6850.059.718011
GTATCAA25000.053.4139821
TCAACGC29650.044.3825874
ATCAACG30200.043.8855443
CAACGCA30350.043.2040865
AACGCAG31400.041.7593656
TATCAAC36700.035.8730742
ACGCAGA39800.032.9458277
GTACATG53500.032.25441
TACATGG53600.031.6678872
CGCAGAG41750.031.4070428
ACATGGG54800.031.046123
GGTAATC5400.029.5913318
ACCTAAG16550.028.9786971
AAGGGTA5550.028.7915655
GTATAGG3600.028.7340471
GCAGAGT46450.028.4315099
GTCTTAG11650.027.444771
GTGCAAG7500.026.9577621
TAGGTAT9250.026.928585