FastQCFastQC Report
Sun 14 Apr 2019
SRR6576868_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576868_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1271238
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA43520.3423434478830872No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC27960.21994307910871133No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC21590.1698344448482503No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG15630.12295101310690838No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG14750.1160286272122136No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC14270.1122527803605619No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA13840.10887025088929059No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA13690.10769029874814944No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC13330.10485841360941067No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA13270.10438643275295421No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC12990.10218385542282406No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT12790.10061058590130252No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6500.062.2004661
GTATCAA25050.051.6099621
TCAACGC29300.043.137414
ATCAACG29750.042.484913
CAACGCA29850.042.3425835
AACGCAG30800.041.189116
TATCAAC35400.035.98942
TAGGTAT8450.035.0310555
CGCAACG1750.034.9039575
CTTAGGT9000.033.4123343
TTAGGTA9400.032.4903874
ACGCAGA39200.032.3628737
CGCAGAG41350.030.5665288
GTACATG49200.030.4813461
TACATGG49800.030.1141032
AGGTATA9700.030.032356
GTCTTAG10950.029.623991
ACATGGG50900.029.447083
ACCTAAG14700.028.7828461
GCAGAGT44750.028.3491549