FastQCFastQC Report
Sun 14 Apr 2019
SRR6576872_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576872_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1315983
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA49950.379564173701332No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC30530.2319938783403737No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC26820.20380202479819268No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG17490.13290445241313908No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC16720.12705331299872416No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG15180.1153510341698943No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA15140.11504707887563896No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA14550.11056373828537298No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC13670.10387672181175593No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT13600.10334480004680913No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC13370.10159705710484102No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6950.060.199561
GTATCAA27200.056.6882481
TCAACGC32700.046.424144
ATCAACG33000.046.0020983
CAACGCA33400.045.451185
AACGCAG34450.044.3387266
TATCAAC41350.036.834742
ACGCAGA41950.036.299627
CGCAGAG43650.034.778228
GTCTTAG9950.032.599741
GTACATG57200.031.8877431
CTTAGGT9450.031.8300443
TAGGTAT10150.031.4870475
AGGTATA9600.031.33276
GCAGAGT49050.031.3326999
TACATGG58950.031.100612
ACATGGG59800.030.4157733
TTAGGTA10600.029.2635614
ACCTAAG16200.029.0183751
TAAGACA19650.028.2233494