FastQCFastQC Report
Sun 14 Apr 2019
SRR6576872_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576872_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1315983
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA50530.38397152546803415No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC30570.232297833634629No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC25370.1927836453814373No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG15360.11671883299404325No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC14270.10843605122558575No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG14040.10668830828361765No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC13680.10395271063531977No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA13470.10235694534047933No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7300.056.1667671
GTATCAA25350.051.4973451
ATCAACG29300.043.7913483
TCAACGC29500.043.6537784
CAACGCA30850.041.894245
AACGCAG32100.040.4092566
TATCAAC37450.034.386822
TACCGAG550.002717797434.180167
ACGCAGA40500.031.7959887
CGCAGAG41900.030.7335938
GTACATG53850.030.456261
GTCTTAG11300.030.4458071
TAGGTAT9800.030.2128185
TACATGG54700.029.9868892
ACCTAAG16750.029.8245641
CTAAGAC17400.029.4422193
ACATGGG55350.029.2950843
CTTAGGT10050.028.9947223
GGGTATA3950.028.6349981
CCTAAGA18600.028.300782
Produced by FastQC (version 0.11.2)