FastQCFastQC Report
Sun 14 Apr 2019
SRR6576873_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576873_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1272937
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA50950.40025547218754737No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC31960.2510729124850641No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC26890.21124376147444845No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC17180.13496347423321028No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG17080.13417788940065378No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG16640.13072131613740506No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA14180.11139592925651466No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC13670.1073894466104764No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA13620.10699665419419814No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT13460.10573971846210772No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT13390.10518980907931814No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC12770.10031918311746771No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7350.062.038361
GTATCAA29050.052.267571
TCAACGC32300.045.968014
ATCAACG33250.044.654643
CAACGCA33600.044.3293275
AACGCAG34450.043.235576
TATCAAC39350.038.466892
GTCTTAG11750.037.6067621
TAGGTAT10450.036.425
CTTAGGT10650.035.294873
TTAGGTA10850.035.0773244
ACGCAGA42600.035.0742767
AGGTATA11100.033.8639956
TCTTAGG13250.033.7041742
CGCAGAG45000.033.2036488
GGTATAG11650.032.2652637
TAAGTCG600.004167093431.3241925
GCAGAGT48250.031.2592729
GGTAATC4950.030.3749778
GTACATG54000.029.9461211
Produced by FastQC (version 0.11.2)