FastQCFastQC Report
Sun 14 Apr 2019
SRR6576880_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576880_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences461769
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6130.13275035786291414No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5300.1147760027199747No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTAA301.4549969E-678.331174
CGCTAAA353.6357142E-667.141015
CCCGCTA451.6126985E-552.2207833
GTATCAA11000.050.0545731
ATCAACG13250.041.146033
TCAACGC14000.039.2774854
CAACGCA14150.038.8611155
AACGCAG14850.036.712796
CCGAACT651.4016466E-436.1998831
TATCAAC15500.035.2189872
TACATGG23000.034.7833672
GTACATG23150.034.3547061
ACATGGG24100.031.5924873
ACGCAGA17500.031.1534257
CGCAGAG17500.030.8848618
TACCGTG804.777278E-429.3741877
CATGGGG9250.028.4532684
GGTATCA5000.028.2359091
CATGGGT5600.026.8564014
ATACGTA350.0088363326.85640124-25
Produced by FastQC (version 0.11.2)