FastQCFastQC Report
Sun 14 Apr 2019
SRR6576882_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576882_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1315045
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA54190.41207715325331074No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC34230.2602952750666327No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC26090.1983962525997209No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC17530.13330342307677684No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC17480.1329232079510587No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG17160.13048983114646268No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT17050.1296533578698828No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG16140.1227334425818128No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT14480.11011030040797083No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA26050.053.4014931
GGTATCA7800.053.0221671
TCAACGC30600.044.375854
ATCAACG31450.043.3259053
CAACGCA32200.042.3167575
AACGCAG32850.041.4794466
TATCAAC35850.038.148132
TAGGTAT9500.036.5997735
TTAGGTA9700.035.8451424
CTTAGGT9700.035.8451423
AGGTATA9700.034.8763546
ACGCAGA39800.034.2361767
GTCTTAG10900.033.199661
CGCAGAG41800.032.598088
GGTATAG10500.032.219117
TATAGTA12200.030.8106239
GTATAGT12100.030.6769398
GCAGAGT45500.030.0505129
ACCTAAG16900.029.199271
GTACATG50700.029.1065731