FastQCFastQC Report
Sun 14 Apr 2019
SRR6576882_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576882_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1315045
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA55950.4254607256785889No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC33630.2557326935580151No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC25180.19147633731165092No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT17020.1294252287944519No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG16650.12661163686413773No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC16630.12645955081385046No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC15450.11748647384690258No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG14870.11307597838857225No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG14100.10722066545251303No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT13580.10326642814504447No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT13210.1004528362147303No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5550.060.1950651
GTATCAA25500.058.86351
TCAACGC28550.050.861144
CAACGCA29250.049.643955
ATCAACG29450.049.4663773
AACGCAG31100.046.8419576
TATCAAC37000.039.75512
ACGCAGA38200.038.135737
CGCAGAG39000.037.3804638
TAGGTAT9100.036.1485065
CTTAGGT9400.034.4949043
GTCTTAG10950.034.377271
GCAGAGT43400.033.6990979
TTAGGTA10250.032.5512924
AGGTATA10250.032.0928236
ACCTAAG16950.031.3692591
CTAAGAC18000.030.8065623
GGTATAG10950.030.0412257
TCTTAGG12850.029.988932
GTACATG49600.029.8830171