FastQCFastQC Report
Sun 14 Apr 2019
SRR6576885_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576885_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1348253
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA42230.31322014488378663No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC28120.20856619640379068No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC24040.1783048137107798No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC15440.11451856587747254No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA15210.11281265459820969No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG15070.11177427381952794No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG14610.10836245126100219No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC14110.10465394847999597No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC14070.10435726825751546No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA13660.10131629597709035No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6550.062.4357031
GTATCAA26350.061.1883281
TCAACGC31250.050.3831674
CAACGCA32150.048.826565
ATCAACG32350.048.6699833
AACGCAG33450.047.0694736
TATCAAC38050.041.6322942
ACGCAGA39850.039.510017
CGCAGAG42550.037.002918
GCAGAGT46100.034.6631979
GTACATG54700.033.6004071
TACATGG55700.032.65962
ACATGGG57750.031.3328153
TTAGGTA8300.030.011554
GTGCAAG7400.028.5848481
GTCTTAG10600.028.3810981
TAGGTAT8550.028.0346245
GTCTTAA4550.027.8937841
GTAATCA5100.027.64669
ACCTAAG16000.027.3223511