FastQCFastQC Report
Sun 14 Apr 2019
SRR6576885_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576885_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1348253
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA44310.32864751645277257No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC29550.21917251435746848No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC23390.1734837600954717No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG15670.11622447715673542No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC14940.11081006309646632No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC14840.11006836254026507No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA14550.10791743092728147No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG14180.10517313886933685No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT13590.10079710558774947No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6800.056.8747251
GTATCAA22050.055.3994641
TCAACGC27550.043.6716844
ATCAACG28400.042.1991233
CAACGCA29000.041.326045
AACGCAG30250.039.929086
TATCAAC34700.035.2148172
ACCTAAG14450.032.9661251
GTACATG49850.031.6006281
ACGCAGA38400.031.4533827
TACATGG50100.031.0507322
TAGGTAT10250.030.72085
CTAAGAC16600.030.2940443
CGCAGAG40400.030.0126138
ACATGGG51900.029.2493843
TTAGGTA10650.029.1256684
GTCTTAG11850.029.0548671
CTTAGGT10900.028.8888233
AGGTATA10600.028.8196746
GGTAATC4250.028.7507978