FastQCFastQC Report
Sun 14 Apr 2019
SRR6576886_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576886_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1299742
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA44010.33860566173902207No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC29280.2252754777486609No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC22990.17688125797273613No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG15590.11994688176576582No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG15450.11886974491860693No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC14590.11225304714320226No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC14500.11156060202717154No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA14480.11140672533472029No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC13590.10455921252063871No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT13240.10186637040274146No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT13240.10186637040274146No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5700.058.5455631
GTATCAA23300.055.4737131
TCAACGC28600.044.0296824
ATCAACG29300.042.8174173
CAACGCA29950.041.8881575
AACGCAG30700.041.017886
TATCAAC35750.035.4975362
GTCTTAG11150.032.4583441
TTAGGTA9950.032.111654
ACGCAGA39100.031.9655137
GTACATG49550.031.5871941
CTTAGGT10250.031.1717993
CGCAGAG40200.031.0908378
TAGGTAT9850.031.0065845
TACATGG50900.030.4723992
ACATGGG52350.028.9909633
TATAGTA12800.027.5313879
ACCTAAG14900.027.443751
GCAGAGT45550.027.4391149
TCTTAGG12850.027.4327032