FastQCFastQC Report
Sun 14 Apr 2019
SRR6576886_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576886_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1299742
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA45270.34829989336345213No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC30770.23673929133628058No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC22820.1755733060869003No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG16190.12456318253930396No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC15210.11702322460919168No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG15160.11663853287806349No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC15160.11663853287806349No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT14650.11271467722055609No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA14510.11163754037339718No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT13550.10425145913573618No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT13160.10125086363293638No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6850.059.1115841
GTATCAA22200.056.4148451
TCAACGC28650.042.3178524
ATCAACG28850.042.024493
CAACGCA29600.040.6421625
AACGCAG30900.039.2364546
TATCAAC36150.033.9281922
GTCTTAG10350.033.6632541
CTTAGGT9550.033.460633
GTACATG52200.033.102461
TACATGG52000.032.894752
TAGGTAT9900.031.8030035
GCATATA902.5970146E-531.3283332
ACATGGG53700.031.1533153
GGTACCT7250.031.1338338
ACGCAGA38900.031.0464577
TTAGGTA10300.030.5679364
CGCAGAG40600.029.9987458
ACCTAAG15450.029.8649851
TCTTAGG12600.029.8365082