FastQCFastQC Report
Sun 14 Apr 2019
SRR6576887_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576887_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1382517
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA43860.3172474551849995No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC29700.2148255681485291No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC22100.15985336889166643No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG16280.11775623735549001No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24300.058.9424131
GGTATCA6800.054.0438541
TCAACGC29800.047.3198434
ATCAACG30200.046.6930923
CAACGCA31350.045.430075
AACGCAG32450.044.324626
TATCAAC36450.038.944642
ACGCAGA42400.034.1397487
CGCAGAG45150.031.8521798
TAGGTAT8600.031.1540625
CTTAGGT8900.030.6320653
GCAGAGT48100.029.8986669
GTACATG55950.029.6417051
GGTAATC4000.029.3734868
TACATGG56600.029.2324052
GTCTTAG10450.028.8552131
TTAGGTA9200.028.6113594
ACCTAAG15100.028.3939021
ACATGGG58400.028.0094573
GTATAGT11100.027.9445068
Produced by FastQC (version 0.11.2)