FastQCFastQC Report
Sun 14 Apr 2019
SRR6576888_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576888_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1295215
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA44360.3424914010415259No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC30870.23833880861478593No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC21990.1697787625992596No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG16490.12731477013468806No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG15260.11781827727442935No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA15050.11619692483487297No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC14130.10909385700443555No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC13990.10801295537806466No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT13850.10693205375169373No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC13760.1062371884204553No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA13630.10523349405311087No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT13010.10044664399346827No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6700.062.45191
GTATCAA25250.051.3899421
TCAACGC27950.045.726294
ATCAACG28550.044.765323
CAACGCA29050.043.994835
AACGCAG30100.042.616236
TATCAAC33700.038.225322
GTCTTAG11750.036.0109941
CTTAGGT10250.033.9224173
GTACATG52050.033.7816851
TACATGG52100.033.6590232
TAGGTAT10250.033.4640055
TTAGGTA10500.033.1147354
ACGCAGA39200.032.723187
CGCAGAG39600.032.2739838
ACATGGG55150.031.2679543
AAGGGTA4700.030.991515
GGTAATC4600.030.6437788
TCTTAGG13250.030.5149732
GCAGAGT43000.029.831369