FastQCFastQC Report
Sun 14 Apr 2019
SRR6576888_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576888_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1295215
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA44410.34287743733665843No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC29250.225831232652494No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC21200.1636793891361666No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG16140.12461251606876078No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC14650.11310863447381322No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA13530.10446142146284594No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG13280.1025312399871836No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT13090.1010643020656802No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC13010.10044664399346827No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7700.066.025791
GTATCAA26700.051.1289981
TCAACGC31000.043.5073284
CAACGCA31600.042.681245
ATCAACG31800.042.4128043
AACGCAG33200.040.9074066
TATCAAC36050.038.3281442
CTTAGGT8650.037.4865263
TAGGTAT8600.036.6115885
TTAGGTA8650.036.3999634
GTCTTAG9850.036.321011
AGGTATA9000.034.462256
GGTATAG9700.033.4287037
ACGCAGA41700.032.568977
GTATAGT10900.031.9263538
CGCAGAG43600.031.2791988
TCTTAGG11950.031.0695482
TATAGTA11300.029.9638869
GTACATG54150.029.6439781
TACATGG54100.029.2757722