FastQCFastQC Report
Sun 14 Apr 2019
SRR6576889_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576889_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1122841
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA17950.15986234916608852No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA17840.15888269131604563No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA17650.15719055502960794No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG15760.14035825196978025No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT15250.13581620193776323No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA14720.13109603229664754No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC14710.13100697249209817No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT13970.12441654695544606No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG13630.12138851360076805No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA13500.12023073614162647No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA12450.11087945666394441No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT12290.10945449979115475No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA12280.1093654399866054No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT12180.10847484194111189No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG11950.10642646643647675No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG11480.10224065562265718No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20050.045.021881
ATCAACG22550.037.723683
CAACGCA23450.036.0754435
TACATGG39100.034.8704872
GTACATG39500.034.6363951
AACGCAG26050.033.9181486
ACATGGG40350.033.428773
TCAACGC26400.032.0442854
TTAGGTA4550.032.020814
ACCTAAG6800.031.8042871
TAGGTAT4650.031.332195
AGGTATA4700.030.9988696
CGTTAAC1551.5133992E-930.3322831
GGTATCA8400.030.223951
TGGGTAC7200.030.026686
CTTAGGT4950.029.433273
ACGCAGA28850.029.323027
CGCAGAG29700.028.4838098
GGGTACC6800.028.33727
CATGGGG26600.027.9162754
Produced by FastQC (version 0.11.2)