FastQCFastQC Report
Sun 14 Apr 2019
SRR6576890_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576890_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1109933
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA18760.169019211069497No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA17790.16027994482549848No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA16610.14964867248743843No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG16360.14739628428022233No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT15220.13712539405531685No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT14060.1266743127738341No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG13740.12379125586859747No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC13710.12352096928373155No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA13640.12289030058571103No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG13520.12180915424624729No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA13430.12099829449164949No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA13330.12009733920876305No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA13120.11820533311470152No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG12720.11460151198315574No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG12180.10973635345556895No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT11970.10784434736150741No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA11430.1029791888339206No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC11310.10189804249445687No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19150.043.0212331
CGTTAAC1357.2759576E-1238.359461
ACCTAAG6650.035.3966331
CAACGCA23200.034.840975
GTACATG40550.034.4809461
ATCAACG23400.034.342353
TACATGG40600.034.2622152
ACATGGG41200.033.535123
AACGCAG25800.031.874886
CCTAAGA7850.031.1303122
TCAACGC26350.030.8542694
GGTATCA9000.030.3388441
CATGGGG24650.029.9317764
TAATAGT3250.028.9198744
GGGTACC7650.028.872627
TGGGTAC7950.027.7818366
ACGCAGA29200.027.6818647
CGCAGAG29900.027.2105858
GTACTAG3650.027.0856951
GGTACCT7850.026.9591278
Produced by FastQC (version 0.11.2)