FastQCFastQC Report
Sun 14 Apr 2019
SRR6576891_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576891_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences430214
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8860.20594401855820593No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6450.14992538597070296No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12600.043.378791
ATCGCGT250.001703143937.5939174-75
GTCCTAC1256.0954335E-933.925211
ATCAACG15800.033.90593
GAACCGT851.7541854E-533.1710976
TCAACGC16550.032.3693774
CAACGCA16800.032.1674085
TACGCTC902.5909916E-531.33189894
TGAACCG1052.0640036E-631.3282595
CATCGCG300.004163705331.32825974-75
AACGCAG17650.030.6182696
GTACATG23150.029.919641
AACCGTG804.7805015E-429.3702417
TACATGG23050.029.3643252
ACATGGG22900.028.728973
TATCAAC19500.028.684072
CATGGGG8000.027.6080274
GCCCGAT605.8483038E-627.41222678-79
GATATAG2600.027.1836591
ACGCAGA19950.026.8527937
Produced by FastQC (version 0.11.2)