FastQCFastQC Report
Sun 14 Apr 2019
SRR6576892_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576892_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1131452
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA41890.37023223256488125No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC28100.24835344318627747No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC21560.1905516097898983No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG15100.13345683245952988No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC14390.12718170987368443No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG13610.12028791323007958No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC13270.11728292494953386No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA12760.11277544252871531No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6300.060.4401931
GTATCAA23300.050.0353741
TCAACGC26600.042.7581754
ATCAACG27200.041.8149763
CAACGCA28300.040.0235865
AACGCAG29000.039.0575036
TATCAAC31350.036.4376262
CTTAGGT9400.035.9989663
TAGGTAT9950.034.4814225
TTAGGTA10150.033.3389434
GTCTTAG11000.033.3336831
ACCTAAG15200.032.473351
ACGCAGA36150.031.3324367
CTAAGAC16900.030.8689373
AGGTATA10900.030.6138026
TCTTAGG12700.030.3522912
GGTATAG11050.030.1982277
GTACATG45200.030.0566671
TACATGG45450.029.5810472
TATAGTA12300.029.4219199
Produced by FastQC (version 0.11.2)