FastQCFastQC Report
Sun 14 Apr 2019
SRR6576892_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576892_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1131452
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA40780.360421829648982No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC28090.24826506117802613No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC21330.18851882360011737No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG14360.1269165638489304No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC12980.11471984671024488No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC12330.10897501617390751No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT12190.10773766805838869No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG12000.10605840990161315No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT11490.10155092748079458No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6350.059.3768271
GTATCAA23550.055.035451
TCAACGC27450.045.8862274
ATCAACG27600.045.466563
CAACGCA28700.044.2152185
AACGCAG29600.043.0296176
TATCAAC32100.039.67842
CTTAGGT9250.036.0750163
ACGCAGA35350.036.0288627
TAGGTAT9550.035.9260445
GTCTTAG10650.035.4030841
AGGTATA10100.034.4350136
TTAGGTA9700.034.4014324
CGCAGAG37350.034.099618
GCAGAGT39950.031.762729
GGTATAG11050.031.0478327
GTACATG44650.030.6109811
TACATGG45100.030.1169642
TCTTAGG12750.029.8582742
GTATAGT13000.029.2827848