FastQCFastQC Report
Sun 14 Apr 2019
SRR6576893_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576893_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1069867
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA40120.3749998831630474No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC29430.27508092127339195No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC21020.19647301954355073No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG15180.1418867952745528No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC13180.12319288285366312No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC13070.12216471767051418No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG13060.12207124810840973No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA11430.10683570948538464No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT11060.10337733568752004No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT11010.10290998787699779No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5450.062.0910951
GTATCAA23050.055.2575531
TCAACGC26600.046.809694
ATCAACG26950.046.2017753
CAACGCA28250.044.0756725
AACGCAG29300.042.6565326
TATCAAC32600.038.493462
ACGCAGA36350.034.2541357
CGCAGAG37300.033.381718
TAGGTAT7850.031.7232555
GGTAATC3300.031.324228
GCAGAGT41000.030.3692119
CTTAGGT7750.030.313763
TTAGGTA8500.029.2973584
GGGTATA3800.028.447071
GTGCAAG6350.028.125691
GTCTTAG8700.028.0916611
GTACATG44550.028.062561
AGGTATA8550.028.0269346
TACATGG44700.027.5478122