FastQCFastQC Report
Sun 14 Apr 2019
SRR6576893_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576893_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1069867
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA41230.3853750045566412No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC27760.25947150440194905No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC21300.1990901672824753No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG13520.12637084796521436No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC12830.11992144818000741No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC12810.11973450905579852No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG12390.1158087874474117No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT11490.10739652685801133No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA11040.10319039656331115No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT10780.10076018794859548No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5850.060.3510361
GTATCAA22050.052.7312051
TCAACGC25200.045.1276254
ATCAACG25400.044.772293
CAACGCA25700.044.432515
AACGCAG26950.042.371636
TATCAAC31000.036.841152
TAGGTAT8800.033.1082885
CTTAGGT8850.032.9212343
ACGCAGA34950.032.6728327
CGCAGAG35100.032.5560268
ACCTAAG13750.031.8390081
TTAGGTA9150.030.8146924
AGGTATA9200.030.647226
GTCTTAG9900.029.956061
GCAGAGT39100.029.2254859
GGTAATC3550.029.1424858
CTAAGAC15600.028.9184023
GGTATAG10000.028.6653697
TCTTAGG11000.028.1993982