FastQCFastQC Report
Sun 14 Apr 2019
SRR6576894_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576894_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1364797
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA53850.39456417327998233No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC35930.26326259509656014No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC29430.21563646461708225No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG18800.1377494235406438No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG18520.1356978363815278No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC18090.1325471846728854No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA16490.12082382947793702No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC15990.11716028097951564No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC15610.1143759841207154No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA15520.11371654539099954No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT15520.11371654539099954No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT15220.11151841629194671No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT14300.10477748705485138No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA14220.10419131929510397No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT14150.10367842250532497No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6600.064.8221361
GTATCAA27550.056.6555331
ATCAACG32650.047.072053
TCAACGC33000.046.7135124
CAACGCA34700.044.2895135
AACGCAG35950.042.7495426
TATCAAC39450.039.088832
GTACCGA550.002717653834.180626
ACGCAGA45600.033.5996977
CGCAGAG47000.032.7988478
CTTAGGT12100.032.239723
TAGGTAT11900.031.9904755
ACCTAAG17850.031.8693351
TTAGGTA11700.031.3322334
GTCTTAG13100.031.222941
CTAAGAC18950.031.0026873
GCAGAGT50450.030.5559169
GTACATG57200.030.082291
TACATGG57050.029.9965672
AGGTATA12550.029.9591086
Produced by FastQC (version 0.11.2)