FastQCFastQC Report
Sun 14 Apr 2019
SRR6576894_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576894_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1364797
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA54540.3996198702078038No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC35940.2633358660665286No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC28370.20786974180042891No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG18000.13188774594316957No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG17650.1293232619942746No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC16450.12053074559806332No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC15620.11444925509068382No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA15180.111225332412073No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT15030.11012626786254659No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC14880.10902720331302017No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT14510.10631617742418836No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT14480.10609636451428307No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7800.055.0171971
GTATCAA29100.051.370991
TCAACGC33450.044.400944
ATCAACG33550.044.26863
CAACGCA34550.043.259385
AACGCAG36450.041.0044366
TATCAAC40800.036.287052
ACGCAGA45350.032.851217
CGCAGAG47100.031.5308448
GTACATG58250.031.0875841
TACATGG57850.031.0356792
ACATGGG61100.029.307923
GCAGAGT50900.029.1768729
TAGGTAT10150.028.2465275
CTTAGGT10500.027.75263
TTAGGTA10650.027.3617174
AGGGTAA5000.027.2602146
GTCTTAG11950.027.2290741
GGTAATC4850.027.1322468
ACCTAAG18550.026.1845421