FastQCFastQC Report
Sun 14 Apr 2019
SRR6576895_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576895_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1290457
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA53650.41574418984902245No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC36620.2837754376937783No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC28960.22441662139846583No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG17750.13754817091929447No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG17580.13623080815556038No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC15830.12266972088182714No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC15390.1192600760815742No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC14890.11538547971765042No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT14700.11391313309935937No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA14260.11050348829910643No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT13890.10763628698980283No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA13800.10693885964429657No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT13510.10469159375322075No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT13210.10236683593486649No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT13070.10128194895296781No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC13000.1007395054620185No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA12930.10019706197106916No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7900.058.9948541
GTATCAA27300.052.42241
TCAACGC31800.044.1850474
ATCAACG32500.043.2333683
CAACGCA33100.042.4496845
AACGCAG34150.041.28216
TATCAAC37850.037.3722042
GTCTTAG12850.036.269211
CTTAGGT11600.035.2445953
TTAGGTA11650.034.689964
TAGGTAT11550.034.583445
AGGTATA11700.034.1400646
ACGCAGA41600.033.776077
TATAGTA13700.033.295499
GTATAGT14250.032.340418
GGTATAG12500.032.3310437
TCTTAGG14250.032.3191032
CGCAGAG43600.032.2492038
TATGCCG1252.3667599E-730.0753865
GCAGAGT47200.029.5902549
Produced by FastQC (version 0.11.2)