FastQCFastQC Report
Sun 14 Apr 2019
SRR6576896_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576896_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1168257
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA29630.2536257005093913No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC17100.146371902757698No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC15820.13541540945185862No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG15660.1340458477886287No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT13790.11803909584962899No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA13350.1142728012757467No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA13160.11264644680066116No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA13120.11230405638485368No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC13000.11127688513743123No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT11990.10263152713829234No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11800.10100517266320681No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5900.066.934151
GTATCAA21250.057.5221181
TCAACGC27550.042.4765784
ATCAACG28000.041.9617653
CAACGCA29050.040.6068535
AACGCAG29750.039.335456
TATCAAC35150.033.4376032
ACCTAAG10550.032.530511
TACATGG48150.031.439822
GTACATG48150.031.342181
CCGCCTA300.004163078531.3314584-85
ACGCAGA37700.031.0405737
CGCAGAG37400.031.038248
ACATGGG49900.030.1384723
CTAAGAC12850.028.8932043
TAGGTAT6300.028.3475045
TAAGACA16000.027.317114
GCAGAGT42850.027.2002339
TTAGGTA6750.027.1539234
CCTAAGA12900.026.9688532
Produced by FastQC (version 0.11.2)