FastQCFastQC Report
Sun 14 Apr 2019
SRR6576896_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576896_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1168257
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA29150.24951701551970154No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC16580.14192082735220074No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG16090.1377265447585591No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC15740.13473062862024368No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT13360.11435839887969856No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA12420.10631222410822276No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT12310.10537065046475219No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA12200.10442907682128161No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA11760.10066278224739934No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6150.065.904081
GTATCAA22700.058.9632571
TCAACGC29150.045.635344
ATCAACG29500.045.2532463
CAACGCA29650.044.8657725
AACGCAG31050.042.6914566
TATCAAC36650.036.5531042
ACGCAGA38100.034.5406657
CGCAGAG38650.034.169288
GCAGAGT44600.029.7149319
ACCTAAG10000.028.748741
GTACATG46400.028.4399641
CTAAGAC11250.028.4125233
TACATGG46850.028.293912
ACATGGG49000.027.148373
TAAGACA15150.026.9935534
GTCTTAG6850.026.8326151
AGAGTAC39250.025.5035410-11
GGTAATC2950.025.4904218
TTAGGTA5600.025.1817914
Produced by FastQC (version 0.11.2)