FastQCFastQC Report
Sun 14 Apr 2019
SRR6576897_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576897_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1128260
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA29160.2584510662435963No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC17660.1565242054136458No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC16420.14553383085459026No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG15130.13410029603105666No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA13430.11903284703880311No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT13020.11539893287008313No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA12840.11380355591796218No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC12150.10768794426816514No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA11810.10467445446971442No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT11800.10458582241681881No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11550.1023700210944286No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT11310.10024285182493398No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7150.056.5331461
GTATCAA22350.056.149321
TCAACGC27350.045.354734
ATCAACG27850.044.3717463
CAACGCA28350.043.754925
AACGCAG29250.042.408616
TATCAAC34550.035.9142382
ACGCAGA36250.034.2193687
CGCAGAG36500.033.9849858
CTAAGAC11750.032.390913
ACCTAAG11100.031.7577021
GCCTAAC600.00416034331.3342671
GTACATG44850.030.9150771
TACATGG45450.030.0933022
GCAGAGT41700.029.7470479
TAAGACA15700.029.3293514
CCTAAGA13000.029.2854842
ACATGGG46400.029.0629653
TGTCGCG650.00616256228.9149694
ACCGTCC3550.027.795028
Produced by FastQC (version 0.11.2)