FastQCFastQC Report
Sun 14 Apr 2019
SRR6576909_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576909_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1234366
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA45170.3659368453116823No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC24810.20099387053758772No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC20500.16607716025878871No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC14280.11568691943880502No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC13950.11301348222488306No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT13210.10701850180578532No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG12440.10078048163996739No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG12380.10029440214652705No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6800.060.2998661
GTATCAA25650.053.2864271
TCAACGC28600.046.509964
ATCAACG29150.045.6342623
CAACGCA30250.044.1266485
AACGCAG31650.042.4717646
TATCAAC35550.038.476592
TAGGTAT7600.035.250825
ACGCAGA39000.034.3441667
CGCAGAG40100.033.167668
CTAAGAC16950.033.0004923
ACCTAAG16050.032.007921
CTTAGGT8450.031.7074493
TTAGGTA8750.031.1562714
AGGTATA8650.030.9718176
GTCTTAG9800.030.2984541
GCAGAGT44000.030.0141759
GGTATAG9150.029.7906287
GGTAATC3950.029.7451178
CCTAAGA18650.029.488332
Produced by FastQC (version 0.11.2)