FastQCFastQC Report
Sun 14 Apr 2019
SRR6576910_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576910_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1245127
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA45680.36687020681424465No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC28050.2252782246309011No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC23010.18480042598064292No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT16860.1354078740562208No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC15970.12826000881837757No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC14940.11998776028469384No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG13420.10778017021556836No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG13400.10761954403044831No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6350.061.438611
GTATCAA26900.055.741081
ATCAACG31750.045.875473
TCAACGC32000.045.5170754
CAACGCA33050.044.070995
AACGCAG33550.043.6942866
TATCAAC36500.040.1773532
ACGCAGA41100.035.4390837
TAGGTAT7250.034.995995
CGCAGAG43250.033.6773728
GCAGAGT46000.031.664059
CTTAGGT7850.030.5255223
GTACATG48600.030.4657121
TTAGGTA8050.030.3507944
GTCTTAG9350.029.6604291
TACATGG50000.029.611482
ACCTAAG14300.029.5830881
ACATGGG50900.028.9850753
CTAAGAC16150.027.6384493
GGTATAG8900.027.4521247