FastQCFastQC Report
Sun 14 Apr 2019
SRR6576910_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576910_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1245127
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA47050.3778731004949696No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC26480.21266906909897546No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC22560.1811863368154413No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT15520.12464591965317595No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC14610.11733742823021266No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC14600.11725711513765262No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG14210.11412490452781122No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG12830.10304169775452625No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22500.056.2609941
GGTATCA7500.051.4505731
TCAACGC28100.043.645914
ATCAACG28300.043.3374563
CAACGCA29600.041.5928735
AACGCAG30400.040.501586
TATCAAC35650.035.4584352
GTACATG49050.032.81141
ACGCAGA37550.032.789567
CGCAGAG38750.031.7984038
TACATGG50350.031.7326432
ACATGGG51500.031.1140463
CTAAGAC16500.030.7573683
GTATATG1701.200533E-1030.4495871
ACCTAAG14850.030.4216271
TAGGTAT8800.029.9029965
GCAGAGT42800.029.0092099
TAAGACA18450.028.7800434
GTCTTAG10500.028.2350731
CATGGGG32250.027.9756954
Produced by FastQC (version 0.11.2)