FastQCFastQC Report
Sun 14 Apr 2019
SRR6576911_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576911_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences931119
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA31490.3381952253149168No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC15630.16786253958946173No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC14900.16002251054913497No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA9900.10632368150580108No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT9720.10439052366024106No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC9720.10439052366024106No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5200.067.8155061
GTATCAA19000.052.2155571
ATCAACG22050.042.629073
TCAACGC22200.042.3410344
CAACGCA23250.040.4288645
AACGCAG23500.040.198766
TATCAAC27550.034.1333962
AAGGGTA3350.033.6706055
ACGCAGA28400.033.2630587
TACATGG36550.031.260072
CGCAGAG30400.031.07478
GTACATG37900.030.7668881
TAGGTAT3850.030.518545
ACCTAAG9400.030.5121671
ACATGGG38450.029.580363
GGTAATC3550.029.125868
AGGTATA4050.029.011456
AATCGGG650.006154503728.92218294
CTTAGGT4150.028.312383
GCAGAGT33300.028.2273589
Produced by FastQC (version 0.11.2)