FastQCFastQC Report
Sun 14 Apr 2019
SRR6576912_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576912_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences949444
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA32870.346202619638441No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC17950.1890580171131736No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC15280.1609362953475929No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT11220.11817442629581101No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC11200.11796377669457071No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA9720.10237570620278817No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4250.058.6103171
GTATCAA17150.054.8091431
TCAACGC20550.045.0453574
CAACGCA21050.043.97545
ATCAACG21250.043.5615123
AACGCAG21950.042.1723026
ACCTAAG9600.036.228271
TATCAAC27200.034.7325942
CTAAGAC11050.033.5939033
ACGCAGA28000.033.0600787
TAAGACA11950.033.029884
CCTAAGA11700.032.1376532
TACATGG34550.031.9690592
CGCAGAG29100.031.810388
GTACATG35400.031.7308561
TTAGGTA5800.031.596014
TAGGTAT5900.030.2640635
CTTAGGT5900.030.2640633
ACATGGG36550.030.2117673
GGTATAG6350.029.5993337
Produced by FastQC (version 0.11.2)